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Physiol Plant. Sagi M, Fluhr R, Lips SH: Aldehyde oxidase and xanthine dehydrogenase in a flacca tomato mutant with deficient abscisic acid and wilty phenotype. Environ Exp Bot. Anal Biochem. Download references. We dedicate this manuscript to the memory of Andrew G. Netting : good friend, gentleman scientist and Antarctic adventurer. You can also search for this author in PubMed Google Scholar. Correspondence to Ian C Dodd. ICD grew plants, collected xylem sap samples and conducted radio-immunoassays.
All surviving authors read and approved the final manuscript. Reprints and Permissions. Netting, A. Xylem sap collection and extraction methodologies to determine in vivo concentrations of ABA and its bound forms by gas chromatography-mass spectrometry GC-MS. Plant Methods 8, 11 Download citation. Received : 23 December Accepted : 22 March Published : 22 March Anyone you share the following link with will be able to read this content:.
Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. View archived comments 1. Skip to main content. Search all BMC articles Search. Download PDF. Abstract Background Accurate quantification of xylem sap ABA concentrations is important to underpin models of root-to-shoot ABA signalling to predict the physiological effects of soil drying.
Results Xylem sap ABA concentrations were highly dependent on the sampling methodology used: the highest concentrations were detected in sap collected by applying an overpressure to detached leaves following the measurement of leaf water potential. Conclusions Although xylem concentrations of these bound ABA forms and ABA-GE suggest they have a limited physiological impact on ABA homeostasis in tomato, the methods developed here will allow a more complete understanding of ABA biochemistry and root-to-shoot signalling in species known to have higher concentrations of these compounds.
Background Roots in drying soil produce chemical signals such as abscisic acid ABA [ 1 ] that can be transported to the shoots to modify their physiology. Figure 1. Full size image. Figure 2. Results Since wild-type plants grew faster than the ABA-deficient mutants, the latter had a smaller leaf area when harvested. Figure 3. Figure 4. Figure 5.
Figure 6. Figure 7. Discussion Ongoing interest in irrigation techniques such as partial rootzone drying has stimulated the measurement and modelling of in planta xylem ABA concentrations [ 14 — 16 ]. Figure 8. Conclusions In species which contain considerable quantities of Adducts I and II [ 33 ], the technique described below allows chemical separation of these compounds from both free ABA and ABA glucose-ester to allow their quantification. Methods Plant culture Tomato Solanum lycopersicum Mill.
Comparison of GC-MS and radioimmunoassay RIA determinations Xylem sap was harvested from the roots and stems of three tomato plants as described above.
References 1. CAS Google Scholar 9. Article Google Scholar Acta Soc Bot Pol 39 : — Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide.
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Assessment of xylem phenology: a first attempt to verify its accuracy and precision. Lupi , C. Lupi 3. Oxford Academic. Cite Cite C. Select Format Select format. Permissions Icon Permissions. Abstract This manuscript aims to evaluate the precision and accuracy of current methodology for estimating xylem phenology and tracheid production in trees. For each sample, the radial number of cells in the cambial zone, radial enlargement phase, cell wall thickening phase and mature cells were counted along three radial rows.
Total number of tracheids was calculated as the sum of the number of cells in radial enlargement, wall thickening and lignification and mature cells. The Gompertz function was defined as follows:. Indeed, if a group has n elements in our case 12 , the number of k combinations in our case two, to compute differences can be calculated using factorials as follows:. Figure 1. Open in new tab Download slide. Figure 2. Table 1. Xylem phenology and cell production estimated in the two study sites.
Onset of xylogenesis DOY Open in new tab. Figure 3. Table 2. Source of variation. Site Figure 4. Widening of xylem conduits in a conifer tree depends on the longer time of cell expansion downwards along the stem.
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